Rat CA153 For Research Use Only Ca153 Elisa Kit Medical Testing

     Principle of the assay

  The kit assay Rat CA153 level in the sample, use Purified Rat CA153 antibody to coat microtiter plate wells, make solid-phase antibody, then add CA153 to wells, Combined CA153 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat CA153 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PREPARATIONS

1.  Allow all specimens and reagents to reach room temperature and mix thoroughly by gentle inversion before use.

2. Prepare Wash Buffer by diluting Wash Concentrate 20-fold with deionized water. The diluted wash solution is stable in room temperature for at least one week.

Specimen requirements

• extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

• Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

PRECAUTION FOR USERS

1.   Handling should preclude any pipetting by mouth. Use only pipettes with disposable tips for each specimen.

2.Do not mix materials from different master lots. Do not use kit components beyond the expiration date.

3.All materials should be brought to room temperature before use.