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Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600

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Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600

Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600
Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600 Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600

Large Image :  Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600

Product Details:
Place of Origin: CHINA
Brand Name: BIOVANTION
Model Number: In-Mo1247
Payment & Shipping Terms:
Minimum Order Quantity: 1 Box
Price: Negotiable
Packaging Details: 96wells/box or 48wells/box
Delivery Time: 5-8 working days
Payment Terms: L/C, Western Union, T/T
Supply Ability: 1000kits/week

Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600

Description
Short Names: IL-13 Method: Sandwich
Cat. No.: In-Mo1247 Gene Names: IL-13
Standard: 135pg/ml Storage: 2-8℃
Highlight:

Mouse Il13 Elisa Kit

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Mouse il 13 elisa kit

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Interleukin 13 Elisa Kit

Mouse Interleukin 13;T-cell activation protein P600; IL-13 Elisa Kit

 

 

  • For research use only.
  • Cat. NO.In-Mo1247
  • For the quantitative in vitro determination of Mouse Interleukin 13(IL-13) concentrations in Mouse serum, plasma, culture media or any biological fluid.
  • Expiration date:6 months .
  • Storage: 2-8℃.
  • Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-13 Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-13 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-13 and HRP conjugated IL-13 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-13. You can calculate the concentration of IL-13 in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

  Materials provided with the kit 48 determinations 96 determinations Storage
1 User manual 1 1 R.T.
2 Closure plate membrane 2 2 R.T.
3 Sealed bags 1 1 R.T.
4 Microelisa stripplate 1 1 2-8℃
5 Standard:135pg/ml 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
6 Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
7 HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
8 Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
9 Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
10 Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
11 Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
12 Wash solution 20ml (20X)×1bottle 20ml (30X)×1bottle 2-8℃

Calculation of Results

Known concentrations of Mouse Interleukin 13(IL-13) Standard and its corresponding reading OD is plotted on the log scale (x-axis) and the log scale (y-axis) respectively. The concentration of Mouse Interleukin 13(IL-13) n sample is determined by plotting the sample’s O.D. on the Y-axis. The original concentration is calculated by multiplying the dilution factor.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1B were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1B were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

 

Assay range

1pg/ml -100pg/ml

 

Sensitivity:

1pg/ml

Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600 0Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600 1Mouse Il13 Elisa Kit Interleukin 13 Elisa Kit T-Cell Activation Protein P600 2

Contact Details
Biovantion Inc.

Contact Person: Mr. Steven

Tel: +8618600464506

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