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Product Details:
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| Short Names: | IL-1 Beta | Specification: | 48wells & 96wells |
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| Method: | Sandwich | Cat. No.: | In-Sh0046 |
| Gene Names: | Il1b | Standard: | 270pg/ml |
| Highlight: | Sheep Interleukin 1 Beta Elisa Kit,IL1F2 ELISA RUO Test Kit,il1b elisa kit |
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Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B;IL1F2 ELISA Kit
Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B ELISA Kit
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1B Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-1B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1B and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1B. You can calculate the concentration of IL-1B in the samples by comparing the OD of the samples to the standard curve.
Materials provided with the kit
| Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
| 1 | User manual | 1 | 1 | R.T. |
| 2 | Closure plate membrane | 2 | 2 | R.T. |
| 3 | Sealed bags | 1 | 1 | R.T. |
| 4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
| 5 | Standard:270pg/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
| 6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
| 7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1B were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay range
3pg/ml -200pg/ml
Sensitivity:
0.5pg/ml
Contact Person: Mr. Steven
Tel: +8618600464506
