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Product Details:
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| Short Names: | IL-8 | Specification: | 48wells/96wells |
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| Method: | Sandwich Method | Cat. No.: | In-Ra1402 |
| Alternative Names: | Granulocyte Chemotactic Protein 1 | Gene Names: | CXCL8 |
| Highlight: | Rat Interleukin 8 Elisa Kit,CXCL8 Elisa Test Kits,IL8 Interleukin 8 Elisa Kit |
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Rat Interleukin 8,IL-8;Chemokine (C-X-C motif) ligand 8,CXCL8 ELisa Kit
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-8. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-8 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-8 and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-8. You can calculate the concentration of IL-8 in the samples by comparing the OD of the samples to the standard curve.
Materials provided with the kit
| Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
| 1 | User manual | 1 | 1 | R.T. |
| 2 | Closure plate membrane | 2 | 2 | R.T. |
| 3 | Sealed bags | 1 | 1 | R.T. |
| 4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
| 5 | Standard:180pg/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
| 6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
| 7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Notes:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-8 were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay range
2 pg/ml -140 pg/ml
Sensitivity:
0.5pg/ml
Contact Person: Mr. Steven
Tel: +8618600464506
