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Product Details:
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| Purpose: | For Research Use Only | Specification: | 48wells/96wells |
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| Method: | Sandwich | Cat. No.: | In-Hu2578 |
| Storage: | 2-8℃ | Package Size: | 48tests/Kit & 96 Tests/Kit |
| Highlight: | 48 Wells Human Osteopontin Elisa Kit,96 Wells Human Osteopontin Elisa Kit,48 Wells osteopontin elisa kit |
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Human Osteopontin,OPN/Secreted phosphoprotein 1/SPP-1 ELISA Kit
Human Chromogranin,CgA ELISA Kit
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Osteopontin (OPN) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Osteopontin (OPN)and HRP conjugated Osteopontin (OPN)antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Osteopontin (OPN). You can calculate the concentration of Osteopontin (OPN)in the samples by comparing the OD of the samples to the standard curve.
Materials provided with the kit
| Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
| 1 | User manual | 1 | 1 | R.T. |
| 2 | Closure plate membrane | 2 | 2 | R.T. |
| 3 | Sealed bags | 1 | 1 | R.T. |
| 4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
| 5 | Standard:13.5ng/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
| 6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
| 7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Notes:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human Osteopontin (OPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HumanOsteopontin (OPN)were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay range
0.1ng/ml-10ng/ml
Sensitivity:
0.01 ng/ml
Contact Person: Mr. Steven
Tel: +8618600464506
