Product Details:
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Package Size: | 48tests/Kit & 96 Tests/Kit | Purpose: | For Research Use Only |
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Specification: | 48wells/96wells | Method: | Sandwich |
Cat. No.: | In-Hu3427 | Storage: | 2-8℃ |
Highlight: | CgA ELISA RUO Test Kit,human chromogranin a elisa kit,Human CgA RUO Test Kit |
Human Chromogranin A,CgA/Pituitary secretory protein I ELISA Kit
Human Chromogranin,CgA ELISA Kit
l Storage: 2-8℃.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Chromogranin A (CHGA) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Chromogranin A (CHGA) and HRP conjugated Chromogranin A (CHGA) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Chromogranin A (CHGA). You can calculate the concentration of Chromogranin A (CHGA) in the samples by comparing the OD of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
1 | User manual | 1 | 1 | R.T. |
2 | Closure plate membrane | 2 | 2 | R.T. |
3 | Sealed bags | 1 | 1 | R.T. |
4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
5 | Standard:540pg/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HumanChromogranin A (CHGA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay range
6pg/ml -400pg/ml
Sensitivity:
1.2pg/ml
Contact Person: Mr. Steven
Tel: +8618600464506