EDTA Blood Collection Tubes

1. Intended Use

Designed for the collection, preservation, and anticoagulation of whole blood for hematological testing, including:

• Complete Blood Count (CBC)

• Hemoglobin (Hb) and hematocrit (Hct) analysis

• Erythrocyte sedimentation rate (ESR) – when combined with specific additives

• Peripheral blood smear preparation

2. Key Features

• Anticoagulant: K₂EDTA or K₃EDTA (dipotassium or tripotassium ethylenediaminetetraacetic acid).

• Volume Options: 2 mL, 3 mL, 4 mL, 5 mL, and 10 mL tubes (standard adult/pediatric sizes).

• Tube Color: Lavender/purple stopper (universal for hematology).

• Sterile & Pyrogen-Free: Complies with ISO 13485 and USP standards.

• Clot Prevention: EDTA chelates calcium ions to inhibit coagulation.

• Stability: Preserves cell morphology for up to 24–48 hours at room temperature (varies by test).

3. Tube Components

• EDTA Concentration: 1.2–2.0 mg/mL blood (optimized for cell integrity).

• Gel Separator (Optional): Inert gel for plasma separation (e.g., for molecular testing).

• Additive-Free Versions: Pure EDTA tubes for standard CBC.

4. Specimen Collection Guidelines

• Fill Volume: Tubes must be filled to the marked line (underfilling causes EDTA excess, leading to cell shrinkage).

• Mixing: Gently invert 8–10 times immediately after collection to prevent clotting.

• Storage:

  • Room Temp (18–25°C): Ideal for CBC within 24 hours.

  • Refrigeration (2–8°C): May alter cell volumes (avoid for smear prep).

5. Compatibility & Interferences

• Tests to Avoid:

  • Calcium, potassium, or iron studies (EDTA interferes).

  • Coagulation tests (use citrate tubes instead).

• Automated Analyzers: Compatible with Sysmex, Beckman Coulter, Abbott, etc.

6. Quality Standards

• Certifications: CE Marked, FDA 510(k) cleared (if applicable).

• Shelf Life: Typically 12–24 months from manufacture (check lot-specific labeling).

7. Ordering Information

• Catalog No.: [e.g., EDTA-5L for 5 mL lavender top].

• Pack Size: 100/pk, 500/pk, or bulk cases.

• Manufacturer: BD Vacutainer®, Greiner Bio-One, Terumo, etc.

8. Safety & Handling

• Biohazard: Dispose of used tubes per medical waste regulations.

• Labeling: Pre-labeled with tracking barcodes (optional).

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