Product Name
Chikungunya Virus (CHIKV) Real-Time RT-PCR Detection Kit
Intended Use
For the in vitro qualitative detection of Chikungunya virus RNA in human serum, plasma, or whole blood samples. Designed for clinical diagnosis, surveillance, and outbreak investigations of Chikungunya fever.
Technical Parameters:
| Product Name: |
Chikungunya Real Time RT-PCR Kit |
| Format: |
Kit |
| Test Type: |
ELISA |
| Applications: |
Medical Diagnosis |
| Specimen: |
Serum 50μl |
| Kit Size: |
96 Tests |
| Sensitivity: |
High |
| Specificity: |
High |
| Storage Temperature: |
2-8°C |
| Shelf Life: |
18 Months
|
Principle of the Test
-
Real-Time RT-PCR: Targets conserved regions of the CHIKV genome (e.g., E1, nsP1, or nsP4 genes) using sequence-specific primers and fluorescent probes (e.g., FAM-labeled).
-
Multiplex Capability: May include an Internal Control (IC) (e.g., RNase P) to monitor RNA extraction and amplification, reducing false-negative results.
Kit Components
-
RT-PCR Master Mix: Contains reverse transcriptase, Taq DNA polymerase, dNTPs, and optimized buffers.
-
Primer/Probe Mix: CHIKV-specific oligonucleotides with fluorescent probes (e.g., FAM for CHIKV, HEX/VIC for IC).
-
Controls:
-
Positive Control: Inactivated CHIKV RNA or synthetic control.
-
Negative Control: Nuclease-free water or negative human matrix.
-
Internal Control (if included).
-
Other Reagents: DNase/RNase-free water, reaction tubes (if applicable).
Workflow
-
Sample Preparation: Viral RNA extraction (compatible with most extraction kits, e.g., QIAamp, MagMAX).
-
PCR Setup: Mix extracted RNA with master mix and primers/probes.
-
Amplification:
-
Reverse Transcription: 50°C for 10–15 min.
-
PCR Activation: 95°C for 2–5 min.
-
Cycling: 40–45 cycles of 95°C (10 sec) → 60°C (30–60 sec, fluorescence read).
-
Analysis: Threshold cycle (Ct) values determined by instrument software.
Performance Characteristics
-
Sensitivity: ≤100 copies/mL (95% LOD).
-
Specificity: No cross-reactivity with Dengue, Zika, Yellow Fever, or other arboviruses.
-
Turnaround Time: ~2 hours (post-extraction).
INTENDED USE
The Chikungunya Real Time RT-PCR Kit is an in vitro nucleic acid
amplification test for qualitative detection of Chikungunya virus RNA
in the biological material (swab, blood plasma, serum, urine) using
real-time hybridization-fluorescence detection of amplified products.
The PCR kit is used for studying the biological material, taken from the
persons suspected of Chikungunya infection without distinction of form
and presence of manifestation.
SUMMARY
Chikungunya virus (CHIKV) is a mosquito-borne virus (arbovirus)
first
found during an outbreak in south Tanzania in 1952. Its
transmission route comes mainly from infected mosquitos of the Aedes
genus, in particular Ae. aegypti and Ae. Albopictus, even if perinatal
vertical transmission has been described as well. Since 1952, CHIKV
caused numerous outbreaks and epidemics in Africa, Asia, Europe, the
South Pacific and Americas, involving millions of people, and often
separated by periods of more than 10 years.
After the bite of an infected mosquito, symptoms of disease occurs
usually between 4 and 8 days but can range from 2 to 12 days.
Chikungunya infection is characterised by an abrupt beginning of chills,
fever reaching up to 40 °C, vomiting, nausea, headache, arthralgia
(joint pain), and in some cases, maculopapular rash. Severe joint and
muscular pain is also observed and is the main and the most
problematic symptom of chikungunya disease. The pain is so intense
that makes movement very difficult and prostrates the patients.
Laboratory diagnosis is generally made by testing serum or plasma
to detect virus, viral nucleic acid, or virus-specific immunoglobulin (Ig)
M and neutralizing antibodies. Viral culture may detect virus in the first
3 days of disease; however, chikungunya virus should be handled under
biosafety level (BSL) 3 conditions. During the first 8 days of disease,
chikungunya viral RNA can often be identified in serum. Chikungunya
virus antibodies normally develop towards the end of the first week of
disease.
Therefore, to definitively rule out the diagnosis,
convalescent-phase samples should be obtained from patients whose
acute-phase samples test negative.
Because of cross-reactivity of Chikungunya antibodies with other
arbovirus, the use of serology is limited and controversial, so the Real
Time RT-PCR is a better detection method.
MATERIALS AND EQUIPMENT REQUIRED BUT NOT SUPPLIED
1. The compatible PCR instruments:
YS-qPCR-1 or SLAN-96P
or ABI 7500 RT-PCR system.
2. Mini centrifuge with rotor for centrifuge tubes.
3. Mini centrifuge with rotor for PCR tubes.
4. Nuclease-free centrifuge tubes.
5. Nuclease-free PCR tubes and caps.
6. Adjustable pipettors and filtered pipette tips for handling DNA.
7. Tube racks.
8. Disposable powder-free gloves.
9. Biological cabinet.
10. Refrigerator and freezer.
11. Biohazard waste container.
STORAGE AND EXPIRATION DATE
1. The kit should be stored at -20℃±5℃.
2. Validity: 12 months. Do not use beyond the expiry date.
3. The production date and expiration date are shown in the
outer package.
FAQ:
Q: What is the brand name of the Elisa Test Kit?
A: The brand name of the Elisa Test Kit is Biovantion.
Q: What is the model number of the Elisa Test Kit?
A: The model number of the Elisa Test Kit is BVTE159.
Q: Where is the Elisa Test Kit made?
A: The Elisa Test Kit is made in China.
Q: Does the Elisa Test Kit have any certifications?
A: Yes, the Elisa Test Kit is certified with ISO13485.
Q: What is the minimum order quantity for the Elisa Test Kit?
A: The minimum order quantity for the Elisa Test Kit is 10.
Q: What is the price of the Elisa Test Kit?
A: The price of the Elisa Test Kit is negotiable.
Q: How is the Elisa Test Kit packaged?
A: The Elisa Test Kit is packaged in a carton.
Q: What is the delivery time for the Elisa Test Kit?
A: The delivery time for the Elisa Test Kit is 7-15 days.
Q: What are the payment terms for the Elisa Test Kit?
A: The payment terms for the Elisa Test Kit are TT 100% payment.
Q: What is the supply ability for the Elisa Test Kit?
A: The supply ability for the Elisa Test Kit is 100,000.
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