AFP ELISA Kit

Intended Use:
The AFP ELISA Kit is a quantitative immunoassay for the detection of Alpha-Fetoprotein (AFP) in human serum or plasma. It is designed for diagnostic and monitoring purposes, including:

• Liver cancer (HCC) screening

• Fetal development disorders (e.g., neural tube defects)

• Germ cell tumor detection

• Pregnancy monitoring (maternal serum screening).

Key Features

• Format: 96-well microplate (strip format).

• Sample Type: Human serum/plasma (EDTA, heparin, or citrate).

• Assay Type: Sandwich ELISA (quantitative).

• Target: Human AFP.

• Detection Method: Colorimetric (OD measured at 450 nm).

• Sensitivity: <1.0 IU/mL (or ng/mL, depending on calibration).

• Dynamic Range: 2–500 IU/mL (or equivalent ng/mL).

• Total Assay Time: ~1–2 hours (varies by protocol).

• Storage: Reagents stable at 2–8°C until expiration.

  Product details	Description
  Delivery	Within 48 hours
  Packaging Specifications	8 x 12 strips, 96 wells
  Country Of Origin	China
  Manufacturer	18 months
  Preservation method	2℃-8℃
  Specimen	Whole blood
  Assification	class1
  Type	Elisa Test Kit

Kit Components

1. Pre-coated Microplate: Anti-AFP antibodies.

2. AFP Standards: Calibrators (0, 5, 25, 50, 100, 250 IU/mL).

3. Enzyme Conjugate: HRP-labeled anti-AFP antibody.

4. Substrate Solution: TMB (Tetramethylbenzidine).

5. Stop Solution: Acidic solution (e.g., 1N H₂SO₄).

6. Wash Buffer: 10–20× concentrated PBS/Tween.

7. Control Samples: Positive/Negative controls (optional).

Workflow Summary

1. Sample Incubation: Add samples/standards to wells (30 min, 37°C).

2. Washing: Remove unbound material.

3. Conjugate Incubation: Add HRP-antibody (30 min, 37°C).

4. Washing: Repeat wash step.

5. Substrate Reaction: Add TMB (15 min, dark).

6. Stop Reaction: Add stop solution.

7. Read Absorbance: Measure at 450 nm (reference: 620–650 nm).

Performance Data

• Precision: Intra-assay CV <5%, inter-assay CV <10%.

• Specificity: No cross-reactivity with CEA, CA-125, or other glycoproteins.

• Accuracy: Recovery rate 90–110% (spiked samples).

Ordering Information

• Catalog Number: [Insert kit code, e.g., AFP-ELISA-96].

• Pack Size: 96 tests (or customizable).

• Regulatory Status: CE-IVD (or for Research Use Only, RUO).

• Storage Conditions: 2–8°C (lyophilized components stable at -20°C).

Applications

• Clinical Diagnostics: HCC surveillance in high-risk patients.

• Prenatal Testing: AFP levels in maternal serum.

• Research: Cancer biomarker studies.