Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Name: Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit
Brand: Biovantion Inc.
SKU: TY0031
Price: 80.00 USD
Availability: InStock

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Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Large Image : Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Product Details:

Place of Origin:	CHINA
Brand Name:	BIOVANTION
Certification:	ISO 13485
Model Number:	TY0031

Payment & Shipping Terms:

Minimum Order Quantity:	1000 Box
Price:	discussible
Packaging Details:	carton/box
Delivery Time:	2-7 days
Payment Terms:	L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability:	100000 Box/carton

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Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Description

Assay Format:	96 - Well Microplate	Sample Types:	Serum, Plasma
Sample Volume:	10 μL (diluted 1:50 In Provided Sample Diluent)	Detection Limit:	18 Months
Storage:	2-8℃	Specimen:	Whole Blood
Assification:	Class1	Product Type:	Elisa Test Kit
Product Name:	Dengue IgM ELISA	Package:	Carton/Box
Highlight:	96 Test Elisa Detection Kit , Human Dengue Virus ELISA Kit , IgM Antibody ELISA Detection Kit

Dengue IgM ELISA

1. Product Overview

The Dengue IgM ELISA Kit is a cutting - edge in - vitro diagnostic solution designed for the rapid and accurate detection of Dengue virus - specific immunoglobulin M (IgM) antibodies in human serum or plasma samples. IgM antibodies typically appear 5 - 7 days after Dengue virus infection, making this kit crucial for early - stage clinical diagnosis and timely patient management. With high sensitivity and specificity, it serves as an essential tool for both clinical laboratories and epidemiological research during Dengue outbreaks.

2. Detection Principle

2.1 μ - Capture ELISA Principle

Antibody Coating: The microplate wells are pre - coated with highly specific anti - human μ - chain antibodies. These antibodies selectively capture any IgM antibodies present in the added samples.

Antigen Binding: Purified Dengue virus antigens (such as envelope protein E or non - structural protein NS1) are added to the wells. If Dengue - specific IgM antibodies have been captured, the antigens will bind to them, forming an antigen - antibody complex.

Enzyme - Labeled Conjugate Binding: A horseradish peroxidase (HRP) - labeled Dengue - specific antibody is then introduced. This conjugate binds to the previously formed antigen - antibody complex, creating a “anti - μ - chain antibody - IgM - virus antigen - enzyme - labeled antibody” sandwich structure.

Color Development and Detection: Addition of the 3,3',5,5' - tetramethylbenzidine (TMB) substrate initiates a color - forming reaction catalyzed by HRP. After stopping the reaction with sulfuric acid (H₂SO₄), the absorbance is measured at 450 nm using a microplate reader. The optical density (OD) value is directly proportional to the concentration of Dengue - specific IgM antibodies in the sample.
3. Kit Components
1. Pre - coated Microplate: 1 x 96 - well plate, pre - coated with anti - human μ - chain antibodies.
1. Calibrators: 6 vials of lyophilized calibrators with known IgM antibody concentrations (0 AU/mL, 5 AU/mL, 10 AU/mL, 30 AU/mL, 80 AU/mL, 200 AU/mL).
1. Positive and Negative Controls: 1 vial each of positive (≥200 AU/mL) and negative (<5 AU/mL) control serum.
1. HRP - labeled Dengue - specific Antibody: 1 bottle (10 mL).
1. Sample Diluent: 1 bottle (30 mL), for sample preparation.
1. Wash Buffer: Concentrated 20x solution (30 mL), to be diluted before use.
1. TMB Substrate: 1 bottle (15 mL) for color development.
1. Stop Solution: 1 bottle (15 mL, 2M H₂SO₄).
1. Sealing Films: 2 pieces for plate incubation.
1. Instruction Manual: Detailed protocol covering sample preparation, assay procedure, quality control, and result interpretation.
4. Performance Characteristics
- Clinical Sensitivity: Effectively detects Dengue - specific IgM antibodies during the acute phase of infection (usually within 2 - 3 weeks post - onset), enabling early diagnosis.
- Clinical Specificity: Significantly reduces false - positive results, ensuring reliable identification of Dengue infections even in regions with co - circulating flaviviruses.
- Reproducibility: Consistent performance across different operators and laboratories, with intra - assay coefficient of variation (CV) ≤8% and inter - assay CV ≤10%.
5. Applications
- Clinical Diagnosis: Early detection of Dengue virus infection, especially useful for patients presenting with acute febrile illness in Dengue - endemic areas. Helps differentiate Dengue from other similar viral diseases.
- Epidemiological Surveillance: Monitoring the spread of Dengue virus during outbreaks, tracking the incidence rate, and guiding public health interventions.
- Research Studies: Investigating the early immune response to Dengue virus, evaluating the efficacy of potential vaccines, and studying the dynamics of IgM antibody production.
6. Precautions and Limitations
- For In - Vitro Diagnostic Use Only: The assay must be performed by trained laboratory personnel in accordance with biosafety regulations.
- Sample Quality: Avoid using hemolyzed, lipemic, or contaminated samples, as they may interfere with test results.
- Result Interpretation: A positive result indicates recent or current Dengue infection but should be confirmed with additional tests (such as IgG detection or viral nucleic acid testing). False - negative results may occur in the very early stages of infection before IgM antibodies reach detectable levels.
- Limitations: The kit may not detect rare Dengue virus strains or extremely low - level IgM antibodies. Cross - reactivity with other flaviviruses may still occur in some cases, requiring careful clinical correlation.

Storage

• Store at 2-8℃.

• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date whichever is earlier.

• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at-20 °C. Avoid multiple freeze-thaw cycles.

• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit 1

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Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

Human Dengue Virus IgM Antibody ELISA Detection Kit 96 Test/Kit

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Human Dengue Virus ELISA Kit

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Dengue IgM ELISA

1. Product Overview​

2. Detection Principle​

2.1 μ - Capture ELISA Principle​

3. Kit Components​

4. Performance Characteristics​

5. Applications​

6. Precautions and Limitations​

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