Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Name: Dengue IgG Antibody ELISA Test System For Serological Diagnosis
Brand: Biovantion Inc.
SKU: TY0027
Price: 80.00 USD
Availability: InStock

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Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Large Image : Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Product Details:

Place of Origin:	China
Brand Name:	Biovantion
Certification:	ISO13485
Model Number:	TY0027

Payment & Shipping Terms:

Minimum Order Quantity:	10
Price:	Negotiable
Packaging Details:	CARTON
Delivery Time:	7-15 days
Payment Terms:	TT 100% PAYMENT
Supply Ability:	100000

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Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Description

Assay Format:	96 - Well Microplate	Sample Types:	Serum, Plasma
Sample Volume:	10 μL (diluted 1:100 In Sample Diluent)	Detection Limit:	15 AU/mL
Sensitivity:	≥96% (confirmed By Clinical Trials On Dengue - Positive Samples)	Specificity:	≥98% (minimal Cross - Reactivity With Other Flaviviruses)
Total Assay Time:	Approximately 1.5 Hours	Storage Conditions:	2 - 8°C, Stable For 18 Months From The Date Of Manufacture

Dengue IgG ELISA

1.Product Overview

The Dengue IgG ELISA Kit is a highly sensitive and specific in - vitro diagnostic tool designed for the qualitative and semi - quantitative detection of Dengue virus - specific immunoglobulin G (IgG) antibodies in human serum or plasma samples. Ideal for both clinical diagnosis and epidemiological research, this kit helps identify past or persistent Dengue infections, complementing IgM - based early - stage detection methods. It is available in a convenient 96 - well microplate format, ensuring efficient high - throughput testing.

2.Detection Principle

2.1 Direct ELISA Principle

Antigen Coating: The wells of the microplate are pre - coated with purified Dengue virus antigens (such as envelope glycoprotein E or non - structural protein NS1).

Sample Incubation: Patient serum or plasma samples are added to the wells. If present, Dengue - specific IgG antibodies in the sample bind to the immobilized viral antigens.

Enzyme - Labeled Conjugate Binding: A horseradish peroxidase (HRP) - labeled anti - human IgG antibody is then added. This conjugate binds to the captured Dengue - specific IgG antibodies, forming an “antigen - IgG - enzyme - labeled antibody” complex.

Color Development and Detection: Addition of the 3,3',5,5' - tetramethylbenzidine (TMB) substrate initiates a color - forming reaction catalyzed by HRP. After stopping the reaction with sulfuric acid (H₂SO₄), the absorbance is measured at 450 nm using a microplate reader. The optical density (OD) value is directly proportional to the concentration of Dengue - specific IgG antibodies in the sample.

3.Kit Components

4.Technical Parameters:

Product Name:	Dengue IgG Antibody ELISA Test
Manufacturer:	Biovantion
Format:	Kit
Applications:	Diagnosis
Assay Time:	2-3 Hours
Sample Type:	Serum, Plasma
Specificity:	High
Sensitivity:	High
Shelf Life:	18 Months
Kit Size:	96 Tests

Dengue IgG Antibody ELISA Test System For Serological Diagnosis 2

5.Performance Characteristics

Clinical Sensitivity: Demonstrated high detection rates in clinical trials, accurately identifying patients with past Dengue infections, especially those with IgG antibody levels above 15 AU/mL.

Clinical Specificity: Minimizes false - positive results, with negligible cross - reactivity against antibodies from other flavivirus infections (e.g., Zika, Japanese encephalitis virus).

Reproducibility: Consistent results across different operators and laboratories, with intra - assay coefficient of variation (CV) ≤8% and inter - assay CV ≤10%.

6. Applications

Clinical Diagnosis: Confirming past Dengue virus infections, especially when IgM results are negative but clinical suspicion remains. Useful for differentiating primary and secondary Dengue infections based on IgG seroconversion patterns.

Epidemiological Surveillance: Assessing the prevalence of Dengue in populations, tracking the spread of the virus over time, and evaluating the effectiveness of vaccination programs.

Research Studies: Investigating the immune response to Dengue virus, studying the kinetics of IgG antibody production, and validating new diagnostic methods.

7. Precautions and Limitations

For In - Vitro Diagnostic Use Only: The assay should be performed by trained laboratory personnel following standard biosecurity protocols.

Sample Handling: Ensure samples are free from hemolysis, lipemia, and contamination, as these may affect test results.

Result Interpretation: Results should be interpreted in conjunction with clinical symptoms, patient history, and other laboratory findings. A positive result does not necessarily indicate current infection.

Limitations: The kit may not detect very low - level IgG antibodies or antibodies from rare Dengue virus strains. False - negative results can occur in the early stages of primary infection when IgG has not yet seroconverted.

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Contact Person: Mr. Steven

Tel: +8618600464506

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Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Dengue IgG Antibody ELISA Test System For Serological Diagnosis

Dengue IgG ELISA

1.Product Overview

2.Detection Principle

3.Kit Components

4.Technical Parameters:

5.Performance Characteristics

6. Applications

7. Precautions and Limitations

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