Diagnostic Kit for Human Parvovirus B19 IgM Antibody (ELISA)

 
 
SUPPORT CUSTOMIZATION: 
 

Biovantion's Elisa test kits can be customized to your needs, making them the perfect solution for your laboratory's needs. Our kits provide highly specific, colorimetric detection methods in a convenient 96-kit. With add-ons like detection buffers and antibody-coated plates, you can be sure of excellent results every time.

Contact BIOVANTION to get the Elisa test kit that satisfies you!
 
PACKAGING:

Elisa test kits are carefully packaged and shipped in an insulated box to ensure the temperature is maintained during transport. The package contains the test kit, instructions, and other supplies needed for the test.

It also includes a return label for customers to return unused portions of the kit after testing is complete. All packages come with tracking information, so customers can track the progress of their shipment.
 
STORAGE OF THE TEST KIT:

Unopened test kits should be stored at 2-8C upon receipt and the microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.
 
ASSAY PROCEDURE: 

• Bring ELISA Kit (all reagents), and samples to room temperature before use (approximately 30 minutes).
• Dilute concentrated wash buffer 1:19 with ddH₂O.
• For each test, set one blank, two positive and three negative controls. Add 100μl positive and negative control serum into positive and negative control wells respectively.
• Add 100μl sample diluent into test wells, then add 10μl specimen in each well.
• Cover wells with seal paper, then incubate 30 minutes at 37°C.
• Wash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for 15 seconds.
• Add 100 μl Enzyme Conjugant to each well except the blank well.
• Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.
• Wash the Plate as step 6.
• Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, include blank. mix well. Cover and incubate at 37℃ for 15 minutes.
• Stop reaction: Add 50μL Stop Solution to each well, include blank mix well.
• Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 630nm to 690nm.

INTERPRETATION OF RESULTS:

• Colorimetric Method
• Cut off Value calculation:
• COV = 0.10 + the average OD of negative controls (If the absorbance of negative controls is below 0.05, calculate it as 0.05.If the absorbance of negative controls is above 0.05; calculate it as its original value.)
• Positive OD450 of sample ≥ COV HPVB19- IgM
• Negative OD450 of sample ＜ COV. HPVB19- IgM