Product Details:
|
Product Name: | AMH Test(Quantitative) | Sample Type: | Serum/plasma |
---|---|---|---|
Type: | Cassette | Package: | 96T |
Storage Temperature: | 2-30℃ | Test Method: | Enzyme-linked Immunosorbent Assay Kit |
Application: | Clinical/ Hospital/ Laboratory/ Home-use | Shelf Life: | 24 Months |
Here’s the content reformatted into a table for better readability:
Section | Details |
---|---|
Product Description | - AMH ELISA is for in vitro quantitative determination of Anti-Mullerian Hormone (AMH) in human serum.- For professional use only. |
Summary | - AMH is a glycoprotein hormone involved in growth differentiation and folliculogenesis.- In males, AMH induces regression of the Müllerian duct, promoting male reproductive tract development.- In females, AMH reflects ovarian reserve and indicates reproductive lifespan.- High AMH (>4.7 ng/ml) may indicate PCOS; low AMH (<1.0 ng/ml) suggests reduced ovarian reserves. |
Applications | 1. Fertility planning.2. Infertility evaluation.3. Ovulatory function assessment.4. Ovarian function monitoring.5. Fertility age prediction. |
Customization | - Brand Name: Biovantion- Model Number: 0081- Place of Origin: China- Certification: ISO13485- Minimum Order Quantity: 50- Price: Negotiable- Delivery Time: 7-15 days- Sample Type: Serum/Plasma- Test Method: ELISA- Shelf Life: 24 months- Storage: 2-30°C. |
Test Principle | - Sandwich principle with an 80-minute duration.- AMH in samples is sandwiched between coated microwells and enzyme-labeled antibodies.- Chromogenic reaction occurs upon substrate addition, proportional to AMH concentration. |
Precautions and Warnings | - For in vitro diagnostic use only.- Handle laboratory reagents with care.- Follow local guidelines for waste disposal.- Avoid using expired reagents or mixing components from different batches.- Handle specimen and waste as biohazard.- Stop Solution contains sulfuric acid; handle with care. |
Storage and Stability | - Store at 2-8°C.- Unused reagents can be stored at 2-8°C for up to 2 months or until the labeled expiry date. |
Specimen Collection & Preparation | - Use human serum.- Store at 18-25°C for up to 8 hours; 2-8°C for 7 days; -20°C for 1 month.- Avoid heat-inactivated samples and grossly hemolytic, lipemic, or turbid samples. |
Calibration | - Standardized against NIBSC code 16/190.- Recalibrate with new reagent lots or when controls fall outside specified ranges. |
Quality Control | - Use controls at low, normal, and elevated ranges.- Test controls alongside patient samples. |
Wash Solution (40X Dilution) | - Dilute 25 mL of Wash Solution Concentrate with 975 mL deionized water to make 1000 mL.- Stable for 2 months at room temperature. |
Test Procedure | - Equilibrate samples and reagents to 18-25°C.- Add 50 µL of calibrators or samples and 50 µL of enzyme conjugate to each well.- Incubate at 37°C for 60 minutes.- Wash wells 5 times with wash solution.- Add 100 µL substrate; incubate in the dark at 18-25°C for 20 minutes.- Add 50 µL stop solution and read absorbance at 450 nm. |
Calculation | - Plot absorbance vs. concentration for calibrators.- Use a best-fit curve (Point-to-Point method) for calibration.- Calculate AMH concentration from the calibration curve. |
Contact Person: Mr. Steven
Tel: +8618600464506