Dengue IgG ELISA
Intended use
The Dengue lgG ELISA is for the qualitative detection of lgG antibodies to dengue antigen serotypes (1, 2, 3 and4) in serum
or plasma, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms and past exposure consistent
with dengue fever. It may also be used to distinguish between primary and secondary dengue infection.
Materials and reagents required but not provided in the kit
1. Pipette capable of delivering 10 µL, 50 µL and 100 µL volumes
2. Microplate reader with a bandwidth of 10 nm or less and an optical density
range of 0-3 OD or greater at 450 nm wavelength is acceptable
3. Absorbent paper for blotting the microwells
4. Timer
5. Distilled or de-ionized water
STORAGE AND STABILITY
Store all components at 2-8°C. Do not freeze. Ensure that the reagents are brought to room temperature before opening.
Return all reagents requiring storage at 2-8 ° C to refrigeration immediately after use. Re-seal the unused microwells in
provided resealable bag with desiccant. All reagents are stable through the expiration date printed on the label if not
opened. Once opened, the kit isstable for 8 weeks at 2-8°C, or until the labeled expiration date, whichever is earlier.
SPECIMEN COLLECTION AND PREPARATION
1. Serum or plasma should be prepared from a whole blood specimen obtained by acceptable venipuncture technique.
2. This kit is designed for use with serum or plasma specimens without additives only.
3. If a specimen is not tested immediately, refrigerate at 2-8°C for up to 3 days. For storage longer than 3 days, the
specimen should be frozen at -20°C. Avoid multiple freeze-thaw cycles. If a specimen is to be shipped, pack in
compliance with federal regulations covering the transportation of etiologic agents.
4. Specimens containing precipitates may give inconsistent test results. Clarify such specimens by centrifugation prior to
performing the assay.
5. Do not use specimens demonstrating gross lipemia, gross hemolysis or turbidity. Do not use specimens containing
sodium azide.
PREPARATION OF THE REAGENTS PRIOR TO ASSAYING
1. Bring all reagents and controlsto room temperature (18-28°C).
2. Preparation of working Wash Buffer:
If precipitants are visible, warm up the Wash Buffer (20X) at 37˚C. Dilute concentrated Wash Buffer (20X) at the rate of 1:20
dilution with distilled water.
TEST PROCEDURE
1.Calculate the desired number of microwells. Remove the remaining microwells and place them with desiccant into the
resealable plastic bag, seal and store at 2-8°C for later use.
2.Add specimens:
2. 1 Blank well: Do not add reagents.
2. 2 Control wells: Add 100 µL Positive Control and Negative Control into the designated control wells.
2.3 Test wells: Add 100 uL Sample Diluent to the test wells, then add 10 uL sample in the corresponding well.
2.4 Gently shake the plate for 20 seconds, and then cover the plate with the sealer.
3. Incubate:Incubate the plate at 37°C for 30 minutes.
4. Wash Step (Can be performed manually or with automated washing):
Manual washing: Carefully remove the incubation mixture by disposing of the solution into a waste container. Fill each
well with 350 µL diluted wash buffer and shake gently for 20-30 seconds. Discard the wash solution completely. Repeat 4
more times. After completing the last wash step, tap the plate on absorbent paper to remove residual liquid.
Automated washing: Automatic plate washer must be calibrated to ensure efficient washing. Fill each well with 350 µL
diluted wash buffer and soak for 20-30 seconds. Aspirate all wells completely. Repeat 4 more times.
5. Conjugate: Add 100 μL conjugate into each well except the Blank Well.
6. Incubate: Cover the wells and incubate at 37°C for 30 minutes.
7. Wash Step: Wash the plate 5 times as described in step 4.
8. Substrate: Add 50 µL of Substrate A and 50 µL of Substrate B into each well, including the Blank Well. Gently shake the
microwellsfor 20 seconds. Incubate at 37°C in the dark for 15 minutes.
9. Stop Solution: Add 50 μL Stop Solution to each well.A yellow color should develop in wells containing Positive specimens.
10. Read Results: Read at 450/630-700 nm within 30 minutes.
Note: Microplate can also be read at 450 nm, but it isstrongly recommended to read it at 450/630-700 nm for better results.
Technical Parameters:
| Product Name: |
Dengue IgG ELISA Kit |
| Manufacturer: |
Biovantion |
| Format: |
Kit |
| Applications: |
Diagnosis |
| Assay Time: |
2-3 Hours |
| Sample Type: |
Serum, Plasma |
| Specificity: |
High |
| Sensitivity: |
High |
| Shelf Life: |
18 Months |
| Kit Size: |
96 Tests |
Specimen collection and preparation
• Human serum or plasma is recommended for this assay.
• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.
• Do not use heat-inactivated samples.
• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.
• Avoid grossly hemolytic, lipemic or turbid samples.
FAQ:
Q: What is the brand name of this test kit?
A: The brand name of this test kit is Biovantion.
Q: Where is this test kit manufactured?
A: This test kit is manufactured in China.
Q: Is this test kit certified?
A: Yes, this test kit is certified with ISO13485.
Q: What is the minimum order quantity for this product?
A: The minimum order quantity for this product is 10.
Q: What is the packaging details for this product?
A: The packaging details for this product is CARTON.
Q: What is the delivery time for this product?
A: The delivery time for this product is 7-15 days.
Q: What are the payment terms for this product?
A: The payment terms for this product is TT 100% PAYMENT.
Q: What is the supply ability for this product?
A: The supply ability for this product is 100000.
Q: Is the price of this product negotiable?
A: Yes, the price of this product is negotiable.
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