This kit is a qualitative detection of human serum/plasma of Human Anti-β 2 Glycoprotein ⅠIgG antibody. The kit is suitable for clinical screening and diagnosis.
β2GP1 is an anti-phospholipid antibody (APL) binding protein, and the binding site of β2GPⅠ and phospholipid is the action site of the APL antibody. Under the action of certain factors, the phospholipid components move to the outer layer of platelets, endothelial cells, and trophoblast cell membranes. The circulating β2GP1 binds to these phospholipid components, and the APL antibody binds to β2GPⅠand produces adhesion molecules, which promote the production of thrombus. β2GPⅠIgG can be used as one of the diagnostic indicators of anti-phospholipid syndrome (APS), spontaneous abortion, and thrombocytopenia.
Dengue virus is a flavivirus found largely in areas of the tropic and sub-tropics. There are four distinct but antigenically
related serotypes of dengue viruses, and transmission is by mosquito,prinicipally Aedes aegypti and Aedes albopictus.
The mosquito-borne dengue viruses (serotype 1-4) cause dengue fever, a severe flu-like illness.The disease is prevalent in
third world tropical regions and spreading to sub-tropical developed countries - including the United States. WHO estimates
that 50-80 million cases of dengue fever occur worldwide each year, including a potentially deadly form of the disease called
dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Primary infection with dengue virus results in a self
limiting disease characterized by mild to high fever lasting 3 to 7 days, severe headache with pain behind the eyes, muscle
and joint pain, rash and vomiting. Secondry infection is the more common form of the disease in many parts of Southeast
Asia and South America. Thisform of the disease is more serious and can result in DHF and DSS. The major clinical symptoms
can include high fever, haemorrhagic evets, and circulatory failure, and the fatality rate can be as high as 40%. Early
diagnosis of DSS is particularly important, as patients may die within 12 to 24 hours if appropriate treatment is not
administered.
Primary dengue virus infection is characterized by elevations in specific NS1 antigen levels 0 to 9 days after the onset of
symptoms; this generally persists upto 15 days. Earlier diagnosis of Dengue reduces risk of complication such as DHF or DSS,
especially in countries where dengue is endemic.
TEST PROCEDURE
1. All reagents should be allowed to reach room temperature for 15 minutes before use.
2. Dilute the wash buffer at the rate of 1:40 dilution with distilled water before use.
3. Add 100μL Sample Diluent in the corresponding well, add 10μL sample in the corresponding well (Do not add in the blank well). Mix thoroughly by using the pipette. Add 100μL of positive control and negative control to the positive control well and negative control well. The sample should be corresponding to the number of micro plate, each plate should be provided with negative control 2 wells, positive control 1 well and blank control 1 well.
Note: Use a separate disposal pipette tip for each specimen, Negative and Positive Control to avoid cross contamination.
4. Shake gently to mix for 30s. Incubate at 37°C for 30 minutes with the sealing plate membrane sealing the plate.
5. At the end of the incubation, remove and discard the plate cover. Take out, add wash buffer to each well for 20 seconds. Repeat 5 times. After the final washing cycle, turn the plate over onto blotting paper or clean towel, and tap it to remove any remainders.
6. Respectively adding Conjugate 50μL (Do not add in the blank well).
7. Incubate at 37°C for 30 minutes with the sealing plate membrane sealing the plate. Repeat the wash step for 5 times as in step 5.
8. Add Substrate A (50μL) and Substrate B (50μL) (Do not add in the blank well). Incubate at 37°C for 10 minutes with the sealing plate membrane sealing the plate.
9. Add 50μL Stop Solution to each well (Do not add in the blank well). Mix gently by shaking, read the absorbance within 10 minutes after stopping the reaction. Calibrate the plate reader with the Blank well and read the absorbance at 450nm. If a dual filter instrument is used, set the reference wavelength at 630nm. Set no blank wells is allowed if use dual wavelength to detect. Calculate the Cut-off value and evaluate the results.
Storage
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date whichever is earlier.
• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at-20 °C. Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
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