IL6 Interleukin-6 For Human Elisa Detect Reagent Elisa Analyzer Research

Product Name

Interleukin-6

Features:

• Product Name: Interleukin-6
• Storage Condition: 2-8℃ Away From Light
• Template Dna: Not Included
• Quality Control: Interleukin-6

Technical Parameters:

Testing Principle

The kit assay Human IL-6 level in the sample, use Purified Human IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells, Combined IL-6 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Specimen requirements

• extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
• Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Storage conditions and shelf life

1. Shelf life: 18 months. 

2. Production date and expiration date are on the package