Product Description:
LH (luteinizing hormone), together with FSH (follicle stimulating hormone), belongs to the gonadotropin family. LH and FSH regulate and stimulate the growth and function of the gonads (ovaries and testes) synergistically. Like FSH, TSH and hCG, LH is a glycoprotein consisting of two subunits (α- and β-chains).1,2,3 This proteohormone, which consists of 121 amino acids and three sugar chains, has a molecular weight of 29500 daltons. In women, the gonadotropins act within the hypothalamus-pituitary-ovary regulating circuit to control the menstrual cycle.4 LH and FSH are released in pulses from the gonadotropic cells of the anterior pituitary and pass via the bloodstream to the ovaries. Here the gonadotropins stimulate the growth and maturation ofthefollicle and hencethe biosynthesis of estrogens and progesterones. The highest LH-concentrations occur during the mid-cycle peak and induce ovulation and formation of the corpus luteum, the principal secretion product of which is progesterone. In the Leydig cells of the testes, LH stimulates the production of test0sterone. Determination of the LH concentration is used in the elucidation of dysfunctions within the hypothalamus-pituitary-gonads system. The determination of LH in conjunction with FSH is utilized for the following indications: congenital diseases with chromosome aberrations (e.g. Turner’s syndrome), polycystic ovaries (PCO), clarifying the causes of amenorrhea, menopausal syndrome, and suspected Leydig cell insufficiency.
Test Principle:
Sandwich principle. Total duration of assay: 80 minutes.
• Sample, Anti-LH coated microwells and enzyme labeled Anti-LH are combined.
• During the incubation, LH presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the LH molecules being sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the LH within the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.
• The absorbance is proportional to the amount of LH in the sample.
Technical Parameters:
| Product Name |
Luteinizing Hormone
|
| Format |
Kit |
| Manufacturer |
Biovantion |
| Test Type |
ELISA |
| Sample Type |
Serum, Plasma |
| Specimen |
Serum 50μl |
| Specificity |
High |
| Sensitivity |
High |
| Storage Temperature |
2-8°C |
| Country of Origin |
China |
This is an Immunoassay Test Kit for Serological Detection, Antigen-Antibody Binding Test Kit for ELISA, and ELISA Assay Kit for Medical Diagnosis.
Applications:
Luteinizing Hormone (LH) has many applications in medicine, especially in the fields of obstetrics, gynecology and reproductive medicine. Here are some of the main applications of LH:
1. **Ovulation monitoring and prediction**:
- The levels of LH in a woman's body change during the menstrual cycle. During ovulation, LH levels rise significantly, and this rise can help determine the best time to conceive. Therefore, measuring LH can help predict the occurrence of ovulation and is especially useful for women trying to conceive.
2. **Infertility Diagnosis and Treatment**:
- People with infertility often have their LH levels tested to help determine whether ovulation is occurring normally. Lower than normal levels of LH may indicate poorly developed follicles or abnormal ovulatory function. For the treatment of infertility, it may be necessary to regulate LH levels to promote ovulation or to adjust fertility treatment regimens.
3. **Diagnosis and Management of Polycystic Ovary Syndrome (PCOS)**:
- PCOS patients often present with abnormal hormone levels, including increased LH levels. LH levels are associated with the development of ovarian cysts, so measuring LH can be used for diagnosis and treatment monitoring of PCOS.
4. **Male Reproductive Health**:
- In men, measurement of LH can be used to assess testicular function and test0sterone synthesis. Lower than normal levels of LH may indicate testicular insufficiency, which may require further evaluation and treatment.
5. **Other clinical applications**:
- LH is also used in the evaluation of a number of other conditions, such as hypogonadism, precocious puberty, and the diagnosis of certain pituitary disorders.
Overall, measurement of LH plays a critical role in the assessment and treatment of a variety of conditions related to reproductive health. Medical professionals can appropriately apply LH testing for diagnosis, prediction, and treatment monitoring based on the patient's specific situation and symptoms.
Support and Services:
The Elisa Test Kit is a diagnostic tool used to detect and measure antibodies or antigens in a sample. It is designed for use in clinical laboratories and is intended for in vitro diagnostic use only.
Our technical support team is available to assist with any questions or issues that may arise during the use of the Elisa Test Kit. We provide troubleshooting assistance, product information, and training resources to ensure that our customers are able to use the product effectively.
In addition to our technical support services, we also offer a range of other services to support the use of the Elisa Test Kit. These include:
- Product training and education
- Validation and verification services
- Custom assay development
- Product customization
- Product consultation and support
Our goal is to provide our customers with the highest level of support and service to ensure the success of their diagnostic testing programs.
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