Product Description:
The Testosterone ELISA Test is a highly sensitive and specific assay designed for measuring testosterone levels in blood samples. It utilizes enzyme-linked immunosorbent assay (ELISA) technology to provide accurate and reliable quantification of testosterone, which is crucial for evaluating hormonal imbalances and diagnosing related conditions. This test is commonly used in clinical settings to monitor hormone levels in both men and women, aiding in the assessment of conditions such as hypogonadism, infertility, and adrenal disorders.
Applications:
The Testosterone ELISA Test is widely applied in clinical diagnostics to measure and monitor testosterone levels. Its primary applications include:
1. **Hormonal Evaluation**: Assesses testosterone levels to diagnose and manage conditions related to hormonal imbalances, such as hypogonadism in men and polycystic ovary syndrome (PCOS) in women.
2. **Infertility Assessment**: Helps in evaluating hormonal causes of infertility by measuring testosterone levels in both men and women.
3. **Adrenal Disorders**: Aids in diagnosing disorders affecting adrenal function, which can influence testosterone production.
4. **Endocrine Disorders**: Used to investigate and monitor various endocrine disorders that impact testosterone levels.
5. **Clinical Research**: Supports research into hormonal function and related health conditions by providing accurate testosterone measurements.
Overall, the Testosterone ELISA Test is essential for accurate hormone assessment and effective management of related health issues.
Instructions:
1. **Sample Collection**:
- Collect blood samples using standard venipuncture techniques.
- Process samples according to the kit's guidelines, usually involving centrifugation to obtain serum or plasma.
2. **Preparation**:
- Allow all kit components and samples to reach room temperature before use.
- Prepare assay buffers and reagents as instructed, following the specific dilution and mixing protocols provided in the kit manual.
3. **Assay Setup**:
- Secure the microplate in the ELISA reader or incubator.
- Add the sample, standards, and controls to the designated wells of the microplate.
- Follow the kit instructions for the amount of sample to be added and the order of addition.
4. **Incubation**:
- Incubate the microplate at the recommended temperature for the specified duration. Ensure proper mixing or shaking if required.
5. **Washing**:
- After incubation, wash the wells multiple times with the provided wash buffer to remove unbound substances.
- Use an automated plate washer if available, or perform manual washing according to the kit instructions.
6. **Detection**:
- Add the enzyme-linked secondary antibody to each well, as directed. Incubate for the recommended time and temperature.
- Add the substrate solution and incubate until the color develops.
7. **Stopping the Reaction**:
- Add the stop solution to halt the enzyme reaction at the specified time.
8. **Reading Results**:
- Measure the optical density (OD) of each well using an ELISA plate reader at the specified wavelength.
- Generate a standard curve using the provided standards and compare the OD of samples against this curve to determine testosterone concentrations.
9. **Data Interpretation**:
- Analyze the results using the provided instructions for calculation and interpretation.
- Verify that the results fall within the expected range for controls to ensure assay validity.
10. **Cleanup**:
- Dispose of all used materials according to safety and biohazard regulations.
- Clean and store equipment as per manufacturer recommendations.
Always refer to the specific instructions provided in the kit’s manual for detailed procedures and troubleshooting tips to ensure accurate results.
Technical Parameters:
| Product Attribute |
Value |
| Product Name |
Detection Kit for Testosterone |
| Applications |
ELISA |
| Assay Time |
1 Hour |
| Sensitivity |
High |
| Format |
Kit |
| Country of Origin |
China |
| Sample Type |
Serum, Plasma |
| Storage Temperature |
2-8°C |
| Kit Size |
96 Tests |
| Shelf Life |
18 Months |
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