Product Description:
Detection Kit for thyroid-stimulating hormone(TSH)(Enzyme-Linked ImmunoSorbent Assay , ELISA).It is used in quantitative tests for thyroid-stimulating hormone(TSH) in human serum.
Thyroid-stimulating hormone (TSH) secretion is controlled by a negative feedback mechanism in which the variations of circulating thyroid hormone levels interact with the hypothalamic- pituitary axis to adjust TSH production.
TSH synthesis and secretion are also stimulated by TRH, a tripeptide produced by the hypothalamus and inhibited by somatostatin.
In the normal population, circulating TSH has been found, using this procedure, to be below 4.5 mIU/L under baseline conditions.
Investigation of a hyperthyroid population demonstrates an increased frequency of samples whose TSH levels are less than 0.1 mIU/L and at the detection limit. Development of sensitive determinations of TSH has therefore facilitated clinical interpretation of hypothalamic, pituitary and thyroid related desorders.
Indeed, elevated levels of T3 and T4 reduce TSH output by the pituitary. However, a single basal serum TSH determination is not considered to be discriminatory as such for the diagnosis of hyperthyroidism, which often requires a dynamic TRH test.
On the contrary, the demonstration of an elevated circulating TSH level is a clear indication for the development of primary hypothyroidism in most instances. In such situation, compl- ementary investigation of the pituitary/thyroid axis should be undertaken. Here again, the most useful dynamic test is the TRH stimulation test.
In case of simultaneous elevation of both TSH and thyroid hor- mone levels, the diagnosis should be oriented towards a state of thyroid hormone resistance and further documented by stimuli- tion(TRH) and inhibition(T3, T4)tests.
Applications:
TSH kit uses a “sandwich principle”, Enzyme-linked immunological sorbent assay. To measure TSH levels in serum, plastic wells coated with a monoclonal antibody of TSH are supplied in the kit. After the patient’s specimen and another mono-antibody labelled with HRP are added, TSH, if present, is fixed to the solid phase antibody and creating a HRP-antibody ~TSH~antibody “sandwich”. After TMB substrate added, the result is obtained by EIA plate reader.
Support and Services:
The Elisa Test Kit is a diagnostic product designed to detect the presence of certain antigens or antibodies in a sample. It is used primarily in medical and research settings to aid in the diagnosis of various diseases or conditions.
Our technical support team is available to assist with any questions or concerns you may have regarding the use of the Elisa Test Kit. We offer a range of services, including:
- Assistance with product installation and setup
- Guidance on sample preparation and test procedures
- Troubleshooting and issue resolution
- Product training and education
- Customization of testing protocols to meet specific needs
We are committed to providing our customers with the highest level of support and service to ensure the accuracy and reliability of their test results.
Packing and Shipping:
Product Packaging:
- Single use, sealed pouches containing all necessary components of the kit
- Kit components include microtiter plate, assay diluent, wash buffer, stop solution, substrate, and positive/negative controls
- Clear instructions for use and interpretation of results included in each kit
Shipping:
- Shipped in a secure, sturdy box to prevent damage during transit
- Shipping materials are designed to maintain the integrity of the kit components
- Shipping labels clearly indicate the contents of the package and proper handling instructions
- Standard shipping times apply unless expedited shipping is requested at an additional cost
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