C-Peptide C-P ELISA Test Kit

Drug Names

Generic Name: C-Peptide C-P ELISA Test Kit

INTENDED USE

Detection Kit for C-peptide(C-P)(Enzyme-Linked ImmunoSorbent Assay , ELISA).It is used in quantitative tests for C-peptide(C-P)in human serum.

PREPARATIONS

• Allow all specimens and reagents to reach room temperature and mix thoroughly by gentle inversion before use.
• Prepare Wash buffer by diluting Wash Concentrate 20-fold with deionized water. The diluted wash buffer is stable in room temperature for at least one week.

RELATED TIPS

• This kit is designated for In-Vitro Diagnostic Use Only.
• Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all water out and block the rim of well on absorbent paper for a few seconds. Filling each well with water and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
• Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.

Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with desiccants to minimize exposure to damp air.

Product details

ASSAY PROCEDURE:

• Mark the microtitration strips to be used. All the calibrators and controls should set

duplicate.

• Dispense 50 μl of Calibratorsas/controls/samples into wells.
• Dispense 50 μl of HRP Conjugate to each well.
• Covered the strips with a plate sealer. Mix it gently by swirling the microtiter plate on flat bench. Incubate the plate at 37°C for 60 minutes.
• Wash each well for 3 times, 10 seconds each time. (See wash procedure).
• Dispense 50 μl of chromogen A to each well.
• Dispense 50 μl of chromogen B to each well.
• Covered the strips with a fresh plate sealer. Mix it gently by swirling the microtiter plate on flat bench. Incubate the plate at 37°C for 15 minutes.
• Dispense 50 μl of stopping solution to each well and mix completely.
• Read the absorbance of the plate within 10 minutes. (See read procedure)

CALCULATION OF RESULTS

• Computer: Use the linear fitting function, the logarithm of each calibrator concentration (Log), as X, take the logarithm of the corresponding absorbance value (Log(OD)) as Y, choose double logarithm (or full Logarithmic) Log-Log fitting the concentration of the serum to be tested is calculated from the fitted line.

  Equation: log OD=B*log [concentration]+A

PERFORMANCE CHARACTERISTICS

• Expected value

Each laboratory should establish its own range of normal values. The value give below are only indicative. For information, the range of basal insulin levels in normal subjects was 0.56~3.25 ng/ml. One hour after meal:2.88~10.1 ng/ml.

• Sensitivity

The detection limit of the assay is approximately 0.25ng/ml.

• Precision

Interassay≤15%

Intraassay≤15%

• Specificity: No cross reaction with INS, Pro-INS.