Human ferritin FE ELISA TEST KIT
INTENDED USE
The T-SPOT.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-) in the vicinity of T cells in human whole blood collected in sodium citrate or sodium or lithium heparin. It is intended for use as an aid in the diagnosis of M. tuberculosis infection. The T-SPOT.TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.
Assay procedure:
Step 1: Prepare all reagents, working standards, Blank and samples as directed in the previous sections.
Step 2: Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the kkkkkdesiccant back into the pouch and seal the ziploc, store unused wells at 4°C
Step 3:Pipette standard 50μl to testing standard well,Pipette Sample dilution 40μl to testing sample well, then add kkkkkktesting sample 10μl (sample final dilution is 5-fold), Pipette sample to wells, don’t touch the well wall as far as kkkkkkpossible, and mix gently.
Step 4: Incubate: Cover with the adhesive strip provided, incubate for 30 min at 37℃.
Step 5: Configurate liquid: Dilute wash solution 30-fold with distilled water.
Step 6: Washing: Uncover the adhesive strip, discard liquid, pipette washing buffer to every well, still for 30s then drain, kkkkkrepeat 5 times.
Step 7: Add enzyme: Pipette HRP-Conjugate reagent 50μl to each well, except blank well.
Step 8: Incubate: Operation with 4
Step 9: Washing: Operation with 6.
Step10: Color: Pipette Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, avoid the light kkkkkpreservation for 15 min at 37℃.
Step 11: Stop the reaction: Pipette Stop Solution 50μl to each well, stop the reaction (the blue change to yellow).
Step 12: Calculate: take blank well as zero. Read absorbance at 450nm after pipette Stop Solution within 15min.
Test procedure
Note: Ensure the patients’ samples, reagents (Conjugate, Incubation Buffer, Calibrators) are
at ambient temperature (18-25 ℃) before measurement. Mix all reagents through gently
inverting prior to use.
• Use only the number of wells required and format the microplates’ wells for each
calibrator and sample to be assayed.
• Add 25 µL of calibrators or samples to each well.
• Add 100 µL of enzyme conjugate to each well.
• Shake the microplate gently for 30 seconds to mix.
• Cover the plate with a plate lid and incubate at 37 ℃ for 60 minutes.
• Discard the contents of the micro plate by decantation or aspiration. If decanting, tap and
blot the plate dry with absorbent paper.
• Add 350 µL of wash solution, decant (tap and blot) or aspirate. Repeat 4 additional
times for a total of 5 washes. An automated microplate strip washer can be used. At the
end of washing, invert the plate and tap out any residual wash solution onto absorbent
paper.
• Add 100 µL of substrate to each well.
• Cover and incubate at ambient temperature (18-25℃)in the dark for reaction for 20
minutes. Do not shake the plate after substate addition.
• Add 50 µl of stop solution to each well.
• Shake for 15-20 seconds to mix the liquid within the wells. It is important to ensure that
the blue color changes to yellow completely.
• Read the absorbance of each well at 450 nm ( using 620 to 630 nm as the reference
wavelength to minimize well imperfections) in a micro plate reader. The results should be
read within 30 minutes of adding the stop solution.
| Product details |
Description |
| Delivery |
Within 48 hours |
| Packaging Specifications |
8 x 12 strips, 96 wells |
| Country Of Origin |
China |
| Manufacturer |
18 months |
| Preservation method |
2℃-8℃ |
| Specimen |
Whole blood |
| Assification |
class1 |
| Type |
Elisa Test Kit |
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