Anti-ACA IgM ELISA
INTENDED USE
This kit is a qualitative detection of human serum/plasma of Anticardiolipin IgM antibody. The kit is suitable for clinical screening and diagnosis. ACA antibody is a group of auto-antibodies against a variety of negatively charged phospholipids, It can be used as one of the diagnostic criteria for antiphospholipid antibody syndrome (including thrombosis), spontaneous abortion, thrombocytopenia and central nervous system (CNS) lesions. ACA IgM can be used as prospective index of spontaneous abortion; Positive ACA was significantly associated with CNS thrombosis in patients with systemic lupus erythematosus (SLE).
PRINCIPLE
This kit uses indirect ELISA principle to detect ACA IgM. Purified ACA antigen is pre-coated on the microplate, the ACA IgM antibody in sample will combine with ACA antigen first, then combine with enzyme-labeled second antibody to form antigen-antibody-antibody complex, and shows blue color in the microplate. This kit is used for the specific detection of ACA IgM in human serum/plasma.
Technical Parameters:
| Product Name: |
ANA Elisa Test KIT |
| Manufacturer: |
Biovantion |
| Format: |
Kit |
| Applications: |
Diagnosis |
| Assay Time: |
2-3 Hours |
| Sample Type: |
Serum, Plasma |
| Specificity: |
High |
| Sensitivity: |
High |
| Shelf Life: |
18 Months |
| Kit Size: |
96 Tests |
This ELISA Diagnostic Test Kit for Infectious Diseases is a high-sensitivity Antibody Detection Assay Kit for ELISA Testing. It provides a highly-specific ELISA Assay Kit for Medical Diagnosis with a shelf life of 18 months.
TEST PROCEDURE
1. All reagents should be allowed to reach room temperature for 15 minutes before use.
2. Dilute the wash buffer at the rate of 1:40 dilution with distilled water before use.
3. Add 100μL Sample Diluent in the corresponding well, add 5μL sample in the corresponding well
(Do not add in the blank well). Mix thoroughly by using the pipette. Add 50μL of positive control and
negative control to the positive control well and negative control well. The sample should be
corresponding to the number of micro plate, each plate should be provided with negative control 2
wells, positive control 1 well and blank control 1 well. Note: Use a separate disposal pipette tip for
each specimen, Negative and Positive Control to avoid cross-contamination.
4. Shake gently to mix for 30s. Incubate at 37°C for 20 minutes with the sealing plate membrane
sealing the plate.
5. At the end of the incubation, remove and discard the plate cover. Take out, add wash buffer to
each well for 20 seconds. Repeat 5 times. After the final washing cycle, turn the plate over onto
blotting paper or clean towel, and tap it to remove any remainders.
6. Respectively adding Conjugate 50μL (Do not add in the blank well) .
7. Incubate at 37°C for 20 minutes with the sealing plate membrane sealing the plate. Repeat the
wash step for 5 times as in step 5.
8. Add Substrate A (50µL) and Substrate B (50µL) (Do not add in the blank well). Incubate at 37°C for
10 minutes with the sealing plate membrane sealing the plate.
9. Add 50μL Stop Solution to each well (Do not add in the blank well). Mix gently by shaking, read the
absorbance within 10 minutes after stopping the reaction. Calibrate the plate reader with the Blank
well and read the absorbance at 450nm. If a dual filter instrument is used, set the reference
wavelength at 630nm. Set no blank wells is allowed if use dual wavelength to detect. Calculate the
Cut-off value and evaluate the results.
FAQ:
Q: What is the brand name of this test kit?
A: The brand name of this test kit is Biovantion.
Q: Where is this test kit manufactured?
A: This test kit is manufactured in China.
Q: Is this test kit certified?
A: Yes, this test kit is certified with ISO13485.
Q: What is the minimum order quantity for this product?
A: The minimum order quantity for this product is 10.
Q: What is the packaging details for this product?
A: The packaging details for this product is CARTON.
Q: What is the delivery time for this product?
A: The delivery time for this product is 7-15 days.
Q: What are the payment terms for this product?
A: The payment terms for this product is TT 100% PAYMENT.
Q: What is the supply ability for this product?
A: The supply ability for this product is 100000.
Q: Is the price of this product negotiable?
A: Yes, the price of this product is negotiable.
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