Product Details:
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Test Type: | ELISA | Storage Temperature: | 2-8°C |
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Specificity: | High | Country Of Origin: | China |
Shelf Life: | 18 Months | Applications: | Medical Diagnosis |
Assay Time: | 1 Hours | Format: | Kit |
Highlight: | Serological Detection ELISA Immunoassay Kit,PRV ELISA Immunoassay Kit,Specificity ELISA Immunoassay Kit |
For PRV (Pseudorabies Virus) ELISA reagent, follow these general steps:
1. **Preparation**: Prepare all reagents according to the manufacturer's instructions. This often includes diluting standards, samples, and conjugates.
2. **Coating**: Add the diluted PRV antigen or antibody to the wells of the ELISA plate. Incubate for the recommended time at the specified temperature.
3. **Blocking**: Add blocking buffer to prevent non-specific binding. Incubate as instructed.
4. **Sample Addition**: Add diluted samples and controls to the wells. Incubate for the recommended time.
5. **Washing**: Wash the plate thoroughly to remove unbound substances.
6. **Detection**: Add the enzyme-linked secondary antibody (conjugate) to the wells. Incubate and wash again.
7. **Substrate Addition**: Add the enzyme substrate to the wells. Incubate and develop color.
8. **Stopping Reaction**: Add the stop solution to halt the reaction after the desired color development.
9. **Reading**: Measure the absorbance using a microplate reader at the specified wavelength.
Always refer to the specific product's manual for precise instructions and concentrations.
PRV (Pseudorabies Virus) ELISA (Enzyme-Linked Immunosorbent Assay) is a diagnostic tool used for detecting and quantifying antibodies or antigens associated with Pseudorabies Virus. PRV is a significant pathogen in swine, and its detection is crucial for managing and controlling outbreaks. Here are some key applications of PRV ELISA:
### **Applications of PRV ELISA:**
1. **Disease Surveillance and Diagnosis:**
- **Detection of Infection**: PRV ELISA is used to detect the presence of PRV antibodies in the blood or serum of animals, indicating current or past infection.
- **Monitoring**: It helps in monitoring the spread of PRV in swine populations and in detecting infections in early stages.
2. **Vaccination Efficacy:**
- **Immunity Assessment**: After vaccination, PRV ELISA can assess the immune response by measuring antibody levels in vaccinated animals.
- **Vaccine Development**: It is used in research to evaluate the effectiveness of new vaccines against PRV.
3. **Outbreak Management:**
- **Control Measures**: During an outbreak, PRV ELISA helps in identifying infected animals and implementing control measures to prevent further spread.
- **Traceback**: It assists in tracing the source of an outbreak by identifying infected herds.
4. **Regulatory Compliance:**
- **Certification**: Used in veterinary diagnostics to certify herds or individual animals as free from PRV before movement or trade.
- **Surveillance Programs**: Helps in fulfilling regulatory requirements for disease surveillance and control programs.
5. **Research and Development:**
- **Pathogen Studies**: Utilized in research to study the pathogenesis of PRV and to develop new diagnostic tools or therapeutic strategies.
- **Epidemiological Studies**: Assists in understanding the epidemiology of PRV, including transmission patterns and prevalence in different regions.
### **How to Use PRV ELISA:**
1. **Sample Collection**: Collect blood or serum samples from animals as per standard procedures.
2. **Reagent Preparation**: Prepare ELISA reagents according to the manufacturer’s instructions.
3. **Assay Performance**: Follow the specific protocol for coating, blocking, sample addition, detection, and reading, as outlined in the reagent manual.
4. **Data Analysis**: Interpret results based on the optical density values obtained, comparing them to standards and controls provided with the kit.
Always refer to the specific product's manual for detailed instructions and ensure compliance with laboratory protocols for accurate results.
Our Elisa Test Kit is a high-quality product designed for the accurate detection of various biological substances. We offer comprehensive technical support and services to ensure that our customers receive the best possible experience when using our product. Our team of experts is available to answer any questions related to product installation, troubleshooting, and maintenance. Additionally, we offer training and certification programs to ensure that users are properly equipped to use our product and achieve accurate results. We are committed to providing exceptional customer service and support to all of our customers.
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Q: What is the brand name of the Elisa Test Kit?
A: The brand name of the Elisa Test Kit is Biovantion.
Q: What is the model number of the Elisa Test Kit?
A: The model number of the Elisa Test Kit is BVTE159.
Q: Where is the Elisa Test Kit manufactured?
A: The Elisa Test Kit is manufactured in China.
Q: Is the Elisa Test Kit certified?
A: Yes, the Elisa Test Kit is certified with ISO13485.
Q: What is the minimum order quantity for the Elisa Test Kit?
A: The minimum order quantity for the Elisa Test Kit is 10.
Q: What is the price of the Elisa Test Kit?
A: The price of the Elisa Test Kit is negotiable.
Q: What are the packaging details for the Elisa Test Kit?
A: The packaging details for the Elisa Test Kit is CARTON.
Q: What is the delivery time for the Elisa Test Kit?
A: The delivery time for the Elisa Test Kit is 7-15 days.
Q: What are the payment terms for the Elisa Test Kit?
A: The payment terms for the Elisa Test Kit is TT 100% PAYMENT.
Q: What is the supply ability for the Elisa Test Kit?
A: The supply ability for the Elisa Test Kit is 100000.
Contact Person: Mr. Steven
Tel: +8618600464506