Product Name
TSH(Quantitative)
Features:
- Product Name: TSH(Quantitative)
- Storage Condition: 2-8℃
- Template Dna: Not Included
- Quality Control: TSH(Quantitative)
Technical Parameters:
| Attribute |
Value |
| Product Name |
TSH(Quantitative) |
| Product Type |
ELISA |
| Size |
96T |
| Reaction Conditions |
Standard |
| Quality Control |
TSH(Quantitative) |
| Package Size |
96 Tests/kit |
| PCR Time |
45min |
| Storage Temperature |
2-8°C |
| Catalog Number |
K1081 |
Testing Principle
TSH kit uses a “sandwich principle”, Enzyme-linked immunological sorbent assay. To measure TSH levels in serum, plastic wells coated with a monoclonal antibody of TSH are supplied in the kit. After the patient’s specimen and another mono-antibody labelled with HRP are added, TSH, if present, is fixed to the solid phase antibody and creating a HRP-antibody ~TSH~antibody “sandwich”. After TMB substrate added, the result is obtained by EIA plate reader.
Thyroid-stimulating hormone (TSH) secretion is controlled by a negative feedback mechanism in which the variations of circulating thyroid hormone levels interact with the hypothalamic- pituitary axis to adjust TSH production.
TSH synthesis and secretion are also stimulated by TRH, a tripeptide produced by the hypothalamus and inhibited by somatostatin.
In the normal population, circulating TSH has been found, using this procedure, to be below 4.5 mIU/L under baseline conditions.
Investigation of a hyperthyroid population demonstrates an increased frequency of samples whose TSH levels are less than 0.1 mIU/L and at the detection limit. Development of sensitive determinations of TSH has therefore facilitated clinical interpretation of hypothalamic, pituitary and thyroid related desorders.
Indeed, elevated levels of T3 and T4 reduce TSH output by the pituitary. However, a single basal serum TSH determination is not considered to be discriminatory as such for the diagnosis of hyperthyroidism, which often requires a dynamic TRH test.
On the contrary, the demonstration of an elevated circulating TSH level is a clear indication for the development of primary hypothyroidism in most instances. In such situation, compl- ementary investigation of the pituitary/thyroid axis should be undertaken. Here again, the most useful dynamic test is the TRH stimulation test.
In case of simultaneous elevation of both TSH and thyroid hor- mone levels, the diagnosis should be oriented towards a state of thyroid hormone resistance and further documented by stimuli- tion(TRH) and inhibition(T3, T4)tests.
Storage conditions and shelf life
1. Shelf life: 18 months.
2. Production date and expiration date are on the package
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