Anti-Mullerian Hormone Elisa Test Kit AMH

INTENDED USE

Intended use The AMH assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of Anti-Mullerian Hormone (AMH) in human serum. For professional use only.

Summary

Anti-Müllerian hormone (AMH) is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis.1 In fetal males, AMH is produced by the Sertoli cells and induces regression of the Müllerian duct and therefore promotes development of the male reproductive tract.2 Infant males have very high levels of AMH (>30 ng/ml) that slowly decreases until post-pubescence where it remains at a low level (<10 ng/ml).3,4 In females, AMH begins to be produced near the time of birth with levels increasing until puberty. After puberty, blood AMH levels decrease until menopause where it becomes nearly undetectable (<0.1 ng/ml). AMH concentration in female blood has repeatedly been linked to ovarian reserve, thereby giving an indication to patients’ remaining reproductive lifespans. Additionaly, high levels of AMH (>4.7 ng/ml, 80% CI) in females are an indication of polycystic ovarian syndrome (PCOS).5 When AMH levels drop below 1.0 ng/ml in females, they are considered to have low ovarian reserves. Patients in these ranges are advised to not delay family planning or to undergo infertility treatments such as in vitro fertilization.5,6 The AMH ELISA kit can be used to monitor the progress of patients’ infertility treatments and approximate the onset of menopause.

Test principle

Sandwich principle.

Total duration of assay: 80 minutes. • Sample, Anti-AMH coated microwells and enzyme labeled Anti-AMH are combined. • During the incubation, AMH presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the AMH molecules being sandwiched between the solid phase and enzyme-linked antibodies. • After washing, a complex is generated between the solid phase, the AMH within the sample and enzyme-linked antibodies by immunological reactions. • Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance. • The absorbance is proportional to the amount of AMH in the sample