Enzyme Immunoassay Reagents Elisa Test Kit for Detection Of Hepatitis C Virus (HCV) Antibodies

Anti-HCV ELISA Kit

Applications:

HCV was previously described as an extraintestinal form of non-A, non-B hepatitis (NANBH), becoming a chronic disease in 50% of cases. Additionally, HCV can be transmitted through intravenous drug use, abuse and household contact. The hepatitis C virus is a single-stranded RNA virus with some structural relationship to the family Flaviviridae. The nucleotide sequence cloned from HCV cDNA provides the basis for constructing recombinant peptides representing putative HCV proteins. Screening blood for anti-HCV antibodies using synthetic or recombinant proteins assists in identifying apparently healthy blood donors with antibodies against HCV; otherwise, they may transmit the virus. This is an enzyme-linked immunosorbent assay that uses recombinant proteins derived from the core region of the HCV virus to detect the presence of HCV antibodies in human serum.

Product Description:

Elisa test kits from Biovantion play a key role in diagnostic testing and scientific research. Its long 18-month shelf life makes it ideal for long-term use, while the colorimetric method used ensures precise and accurate results. Each kit can perform 96 tests, and the testing time is 2-3 hours. Biovantion Elisa test kits are trusted by laboratories, clinics and research centers around the world.

Elisa test kits have become the standard for reliable detection of a wide range of components in biological samples. Its broad applications include disease diagnosis, detection of toxins and allergens, and quantification of various molecules. The sensitivity and specificity of the Elisa test kit make it the first choice for laboratory diagnostics.

Elisa test kits are easy to operate with minimal preparation and are suitable for a wide range of applications, including medical pharmaceuticals, food and beverages, and agriculture. Its convenient size makes it ideal for laboratories of all sizes.

Overall, Biovantion's Elisa test kit is an indispensable must-have for any laboratory due to its reliable and accurate detection, ease of use, and broad applicability.

Principle of the Assay:

Multiple epitopes of HCV proteins(Core, NS3, NS4 and NS5) are bound to the microtiter wells. When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase. Non-reactive antibodies are removed with the wash buffer. Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate. Positive sample generates a medium to dark blue color. No color or very pale blue color indicates a negative reaction. The intensity of the reaction is photometrically quantitated.

Materials required but not provided:

• Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
• Disposable pipette tips.
• Distilled water.
• Humidified Box capable of maintaining 37°C
• Absorbent paper or paper towel.
• Microtiter plate or strip-well washer
• Microtiter plate reader.

Support customization:

Biovantion's Elisa test kits can be customized to your needs, making them the perfect solution for your laboratory's needs. Our kits provide highly specific, colorimetric detection methods in a convenient 96kit. With add-ons like detection buffers and antibody-coated plates, you can be sure of excellent results every time.

Contact BIOVANTION to get the Elisa test kit that satisfies you!

Packaging:

Elisa test kits are carefully packaged and shipped in an insulated box to ensure temperature is maintained during transport. The package contains the test kit, instructions, and other supplies needed for the test.

It also includes a return label for customers to return unused portions of the kit after testing is complete. All packages come with tracking information so customers can track the progress of their shipment.

Storage of test kit:

Unopened test kits should be stored at 2-8C upon receipt and the microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.

Assay procedure: 

It is strongly advised to analyze each specimen and controls in duplicate. All the reagents should equilibrate to room temperature before use.

1. Dispense 100ml(or 3 drops) of specimen diluent into individual test wells.

2. Dispense 100ml positive control and negative control duplicate into individual wells.

3. Add 10ml of each test sample into duplicate test wells; vortex to mix.

4. Incubate for 30 minutes at 37°C

5. Wash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.

6. Add 100ml of Enzyme Conjugate to each well. Mix it gently by swirling the microtiter plate on flat bench for 1 minutes. Do not add Enzyme Conjugate to the blank well.

7. Incubate for 20 minutes at 37°C

8. Wash the plate 5 times as step 5.

9. Add one drop (50ml) of Substrate Solution A (HRP-substrate) to each well, then add one drop (50ml) of Substrate Solution B (TMB) to each well. Mix gently and incubate at 37°C for 10 minutes. .

10. Add one drop (50ml) of Stop Solution to each well to stop the color reaction. Read the OD value at 450 nm/630 nm with dual filter plate reader. It is option to read the OD value at 450 nm with single filter plate reader. (using the OD value of the blank well to correct all the OD reading from all wells)