Human Anti-Mullerian Hormone AMH ELISA Kit

Intended use

The AMH assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of Anti-Mullerian Hormone (AMH) in human serum. For professional use only.

Test principle

Sandwich principle. Total duration of assay: 80 minutes.

• Sample, Anti-AMH coated microwells and enzyme labeled Anti-AMH are combined.
• During the incubation, AMH presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the AMH molecules being sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the AMH within the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.
• The absorbance is proportional to the amount of AMH in the sample.

Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis.(1) In humans, the gene for AMH is AMH, on chromosome 19p13.3,(2) while the gene AMHR2 codes for its receptor on chromosome 12.(3)

AMH can be used for selection of females in multi-ovulatory embryo transfer programs by predicting the number of antral follicles developed to ovulation.(4)
AMH can also be used as a marker for ovarian dysfunction, such as in women with polycystic ovary syndrome (PCOS).