Product Details:
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Specimen: | Serum | Read Time: | 110 Minutes |
---|---|---|---|
Storage: | 2-8℃ | EXP: | 24 Months |
Size: | 96 Test/Kit | ||
Highlight: | FT3 Elisa Test Kit,Free Triiodothyronine T3 Free Blood Test,FT3 Elisa Test Kit 110 Minutes |
FT3
FT3 ELISA TEST KIT
Free Triiodothyronine 96 tests
1. Intended use
Immunoassay for the in vitro quantitative determination of free triiodothyronine in human serum.
2. Summary
3. Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with T3 derivant.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 2(B), 5(C), 10(D), 20(E) and 50(F) pmol/L.
• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase) labeled sheep monoclonal Anti-T3 in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with a precision of better than 1.5%.
• Absorbent paper.
• Distilled water.
4. Test Produce
Ensure the patients’ samples, calibrators, and controls are at ambient temperature (18-25 ℃) before measurement. Mix all reagents through gently inverting prior to use.
• Use only the number of wells required and format the microplates’ wells for each calibrator and sample to be assayed. • Add 50 µL of calibrators or samples to each well.
• Add 50 µL of enzyme conjugate to each well.
• Shake the microplate gently for 30 seconds to mix.
• Cover the plate with a plate lid and incubate at 37 ℃ for 60 minutes.
• Discard the contents of the micro plate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper.
• Add 350 µL of wash solution, decant (tap and blot) or aspirate. Repeat 4 additional times for a total of 5 washes. An automated microplate strip washer can be used. At the end of washing, invert the plate and tap out any residual wash solution onto absorbent paper. • Add 100 µL of substrate to each well.
• Cover and incubate at ambient temperature (18-25℃)in the dark for reaction for 20 minutes. Do not shake the plate after substate addition. • Add 50 µL of stop solution to each well.
• Shake for 15-20 seconds to mix the liquid within the wells. It is important to ensure that the blue color changes to yellow completely. • Read the absorbance of each well at 450 nm ( using 620 to 630 nm as the reference wavelength to minimize well imperfections) in a micro plate reader.The results should be read within 30 minutes of adding the stop solution.
Contact Person: Mr. Steven
Tel: +8618600464506