Pro-BNP Elisa Test Kit 

INTENDED USE

This kit a lows for the determination of pro-BNP concentrations in Human serum, ce l culture supernates and other biological fluids

Specimen requirements

1. extract as soon as possible after Specimen colection,and according to the relevant 1 literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in-20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Steps description

Standard, Sample diluent Add Standard, Sample diluent, incubate for 30 min at 37℃. Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃. Wash 5 times,Add Chromogen SolutionAand B, incubate for 10 min at 37℃. Add Stop Solution Read absorbance at 450nm within 15 min calculate

Important notes

1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2. washing buffer wil Crystalization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Voley .

4. if the testing material content is excessively higher (The sample OD is bigger than the first standard we l ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).

5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6. Thesubstrate evade the light preservation.

7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8. Al samples, washing buffer and each kind of reject should according to infective material process.

9. Donotmix reagents with those from other lots.