Product Details:
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Key Words: | Enzyme-linked Immunosorbent Assay | Storage Temperature: | 2-8°C |
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Detection Method: | Colorimetric | Size: | 96T |
Expiry Date: | 18 Months | Method: | ELISA-Sandwich Method-Two Steps |
Incubation: | 37 ℃/ 1.0+0.5hrs | Single Package Size: | 15X15X5 Cm |
Specimen: | Serum/plasma | Usage: | In Vitro Diagnosis, Early For Hepatitis C Screening, Diagnostic Or Research |
Anti-HCV ELISA Kit
Here’s the content presented in a clear tabular format:
Section | Details |
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Applications | - HCV, previously described as an extraintestinal form of non-A, non-B hepatitis (NANBH), becomes chronic in 50% of cases.- Transmitted through intravenous drug use, abuse, and household contact.- Screening blood for anti-HCV antibodies assists in identifying healthy blood donors and preventing virus transmission. |
Product Description | - Biovantion ELISA test kits are essential for diagnostics and research.- Features an 18-month shelf life and precise colorimetric methods.- Performs 96 tests in 2-3 hours.- Widely used in clinical diagnostics, toxin detection, and quantification of biological molecules.- Easy to use with minimal preparation. |
Principle of the Assay | - Multiple epitopes of HCV proteins (Core, NS3, NS4, NS5) are bound to microtiter wells.- Test samples react with recombinant proteins; non-reactive antibodies are washed off.- Bound human IgGs react with anti-human IgG peroxidase conjugate and chromogenic substrate.- Positive reactions produce a blue color, quantified photometrically. |
Materials Required | - Precision pipettes (0.02 to 1.0 ml)- Disposable pipette tips- Distilled water- Humidified box (37°C)- Absorbent paper- Microtiter plate or strip-well washer- Microtiter plate reader |
Support Customization | - Kits are customizable for specific laboratory needs.- Includes detection buffers and antibody-coated plates for excellent results. |
Packaging | - Kits are shipped in insulated boxes to maintain temperature.- Includes test kit, instructions, and return label for unused portions.- Packages have tracking information for shipment progress. |
Storage of Test Kit | - Store unopened kits at 2-8°C.- Keep microtiter plates in a sealed bag to minimize exposure to damp air.- Use reagents promptly after unpacking. |
Assay Procedure | 1. Equilibrate reagents to room temperature.2. Dispense 100 µL of specimen diluent into each test well.3. Add 100 µL of positive and negative controls in duplicate.4. Add 10 µL of test samples in duplicate; mix.5. Incubate for 30 minutes at 37°C.6. Wash wells 5 times.7. Add 100 µL of Enzyme Conjugate; mix gently.8. Incubate for 20 minutes at 37°C.9. Wash wells 5 times.10. Add 50 µL Substrate Solution A and 50 µL Substrate Solution B to each well; mix and incubate for 10 minutes.11. Add 50 µL Stop Solution; read OD values at 450 nm/630 nm. |
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Warranty | 1 Year |
After-sale Service | Online technical support |
Incubation | 37 ℃/ 1.5hrs |
Sample volume | Serum or Plasma |
Specification | 96T |
Contact Person: Mr. Steven
Tel: +8618600464506